An improved method for murine laser-induced choroidal neovascularization lesion quantification from optical coherence tomography images

•Choroidal neovascularization progression can be studied through L-CNV and quantification of lesions on OCT.•A previous method of OCT quantification was novel and simple, yet required many days to evaluate many lesions.•The new, semi-automated method is efficient and much less time-consuming. Laser-induced choroidal neovascularization (L-CNV) in murine models is a standard method for assessing therapies, genetics, and mechanisms relevant to the blinding eye disease neovascular or “wet” age-related macular degeneration. The ex vivo evaluation of these lesions involves confocal microscopy analysis. In vivo evaluation via optical coherence tomography (OCT) has previously been established and allows longitudinal assessment of lesion development. However, to produce robust data, evaluation of many lesions may be required, which can be a slow, arduous process. A prior, manual method for quantifying these lesions as ellipsoids from orthogonal OCT images was effective but time consuming. We therefore developed an OCT lesion quantification that is simplified, streamlined, and less time-consuming. [Display omitted]