First-time report on compound isolation from two Colocasia species: vegetable-derived bioactive metabolites and their medicinal potential

Schott and Hook.f. are two commonly found vegetable species of the genus , found mainly in the Asian region. The objective of this study was to isolate bioactive phytochemicals from and and elucidate their structure employing the NMR technique followed by bioactivity evaluation. Column chromatography was utilized to isolate phytochemicals, followed by NMR analysis for characterization. An analgesic test was performed through an acetic acid-induced writhing test, an anti-inflammatory test was performed through a formalin-induced licking test, and an antidiarrheal test was performed through a castor oil-induced diarrhea model. The antimicrobial test was executed through the disc diffusion method. Computer-aided simulation was also implemented to validate the wet laboratory results. Six compounds from and were isolated and characterized from the dichloromethane (DCM)-soluble fractions of the methanolic extracts of these two species. Three of the compounds were from and proposed as penduletin , a mixture of α-amyrin , β-amyrin , and monoglyceride of stearic acid while the remaining three compounds were from and proposed as penduletin (which was also isolated from ), 7,8-(3″,3″-dimethyl-pyrano)-4'-hydroxy flavonol , and lastly a mixture of 7,8-(3″,3″-dimethyl-pyrano)-4'-hydroxy flavonol and 4',7,8-trihydroxy flavonol . These compounds showed promising zones of inhibition against Gram-positive and Gram-negative bacteria and fungi. In the antidiarrheal test, demonstrated the highest reduction in castor oil-induced diarrhea (44.44%) at a dose of 20 mg/kg compared to loperamide's 77.78% reduction. However, the analgesic assessment showed a noteworthy inhibition of acetic acid-induced writhing by and (56.52%) at a dose of 20 mg/kg compared to the 76.09% by diclofenac sodium. In comparison, showed pronounced anti-inflammatory action by 68.15% and 52.06% reduction, respectively, in the early and later phases compared to the ibuprofen's outcomes of 73.54% and 74.68%. Plausible targets such as dihydrofolate reductase (DHFR) for antimicrobial, kappa opioid receptor (KOR) for antidiarrheal, and cyclooxygenase 2 (COX-2) for anti-inflammatory and analgesic activities showed a noteworthy binding affinity with isolated compounds, and ADME/T studies displayed these phytochemicals' drug-likeness profiles. To the best of our knowledge, this is the first report on compound isolation from these plants, which demands further extensive research for more absolute findings.