Forensic Validation and Application Evaluation of IDentifier DNA Typing Kit （Yan-Huang34）

Objective To investigate the technical performance of IDentifier DNA typing kit （YanHuang34） and evaluate its forensic application value. Methods Following the Criterion of Forensic Science Human Fluorescence STR Multiplex Amplification Reagent （GB/T 37226—2018）, IDentifier DNA typing kit （YanHuang34） was verified in 11 aspects of species specificity, veracity, sensibility, adaptability, inhibitor tolerance, consistency, balance, reaction condition verification, mixed samples, stability and inter batch consistency. The system efficiency of IDentifier DNA typing kit （YanHuang34） was compared with the PowerPlex® Fusion 6C System, VersaPlex® 27PY System and VeriFilerTM Plus PCR Amplification Kit. The IDentifier DNA typing kit （YanHuang34） was used to detect the swabs of biological samples in daily cases and the STR performances were observed. Results IDentifier DNA typing kit （YanHuang34） had good species specificity, veracity, adaptability, inhibitor tolerance and balance. The sensibility was up to 0.062 5 ng.