Isolation and identification of bacteria from fresh guava ( Psidium guajava ) sold at local markets in Mymensingh and their antibiogram profile

The study was conducted for the isolation, identification, and antibiogram of bacteria obtained from fresh guava ( ). A total of 25 fresh guavas were collected from five markets located in Mymensingh city. Guava samples were cultured onto various selective media such as eosin methylene blue, xylose lysine deoxycholate, thiosulfate-citrate-bile salts-sucrose, blood agar, and mannitol salt agar for the isolation of bacteria. Biochemical tests (dextrose, maltose, lactose, sucrose, mannitol, methyl red, Voges-Proskauer, and indole) were performed to identify the bacteria. Total viable counts of guava were ranged between log 6.56 colony-forming unit (cfu)/ml and 6.62 cfu/ml. A total of 106 bacterial isolates belonged to five genera ( spp., spp., spp., and spp.) were identified. spp. (23.6%) was the most prevalent, followed by (22.64%), spp. (19.81%), spp. (17.92%), and spp. (16.03%). The results of antibiotic sensitivity test showed that spp., spp., and were sensitive to chloramphenicol, ciprofloxacin, and gentamicin and resistant to ampicillin and cephalexin. spp. was sensitive to chloramphenicol and gentamicin, intermediately sensitive to ciprofloxacin and ampicillin and resistant to cephalexin. The results of this study indicate that fresh guava contains multidrug-resistant bacteria which might pose a public health risk.