Ecofriendly analytical quality by design-based method for determining Metronidazole, Lidocaine and Miconazole using RP-HPLC in semisolid dosage form

The article deals with the development of an analytical method based on AQbD and GAC for the simultaneous determination of Metronidazole (MET), Lidocaine (LID) and Miconazole (MIC) in oval dosage form using RP-HPLC. The separation was achieved on the stationary phase Zorbax C18 150 mm × 4.6 mm (i.d); 5 μm, using a gradient mobile phase containing Ethanol and Phosphate buffers. The linearity has been obtained for MET's 240-390 μg/mL, 32-52 μg/mL for LID and 64-104 μg/mL for MIC, with their LOD and LOQ as 31.25, 94.705 μg/mL (MIC) 1.463, 4.433 μg/mL (LID) and 7.981, 24.187 μg/mL for MIC, respectively. Finally, the method assessed for greenness using the three tools showed that the developed method was eco-friendly. The developed method is simple and reliable with a design space that helps for further improvement and is eco-friendly, making this method unique for adoption in regular quality control analysis.