Experimental study of O-GlcNAc on the influence of the upper lip development through inhibition of Wnt signa-ling pathway

Objective To explore the role of Wnt signaling pathway in the process of epithelial layer's disappearance when Oxydiethanoldiethylene GlcNAc influence development of upper lip. Methods The over/lower-expression of O-GlcNAc and reverse-high expression experimental group was constructed by transplanting beads into the upper lip of chicken embryo which contain O-GlcNAc promoter OGT (O-GlcNAc transferase), O-GlcNAc inhibitor OGA (O-GlcNAcase) or OGA inhibitor STZ(streptozoticin). and kept incubation for 24 h. Phenotypic analysis of skeletal and morphological changes were performed by using Alizarin Red/Alcian Blue method. TUNEL assay was performed to analyze the cell apoptosis. Immunohistochemistry method was performed to test the Wnt signaling pathway including GSK-3β, β-catenin. Results In the group OGZ and STZ where O-GlcNAc was overexpressed, disappearance of the epithelial layers was delayed, embryo lips appeared bone defect in bonestaining, compared with the normal group, obvious cell apoptosis has not been