Lunar gravity prevents skeletal muscle atrophy but not myofiber type shift in mice

Skeletal muscle is sensitive to gravitational alterations. We recently developed a multiple artificial-gravity research system (MARS), which can generate gravity ranging from microgravity to Earth gravity (1 g ) in space. Using the MARS, we studied the effects of three different gravitational levels (microgravity, lunar gravity [1/6 g ], and 1 g ) on the skeletal muscle mass and myofiber constitution in mice. All mice survived and returned to Earth, and skeletal muscle was collected two days after landing. We observed that microgravity-induced soleus muscle atrophy was prevented by lunar gravity. However, lunar gravity failed to prevent the slow-to-fast myofiber transition in the soleus muscle in space. These results suggest that lunar gravity is enough to maintain proteostasis, but a greater gravitational force is required to prevent the myofiber type transition. Our study proposes that different gravitational thresholds may be required for skeletal muscle adaptation. Artificial lunar gravity is shown to be sufficient to suppress microgravity-induced soleus muscle atrophy but not myofiber type shift in mice.