Host relatedness and landscape connectivity shape pathogen spread in the puma, a large secretive carnivore

Urban expansion can fundamentally alter wildlife movement and gene flow, but how urbanization alters pathogen spread is poorly understood. Here, we combine high resolution host and viral genomic data with landscape variables to examine the context of viral spread in puma ( Puma concolor ) from two contrasting regions: one bounded by the wildland urban interface (WUI) and one unbounded with minimal anthropogenic development (UB). We found landscape variables and host gene flow explained significant amounts of variation of feline immunodeficiency virus (FIV) spread in the WUI, but not in the unbounded region. The most important predictors of viral spread also differed; host spatial proximity, host relatedness, and mountain ranges played a role in FIV spread in the WUI, whereas roads might have facilitated viral spread in the unbounded region. Our research demonstrates how anthropogenic landscapes can alter pathogen spread, providing a more nuanced understanding of host-pathogen relationships to inform disease ecology in free-ranging species. Nick Fountain-Jones et al. use genomic and spatial data to examine how urbanization affects the spread of the pathogen feline immunodeficiency virus in pumas from areas of varying anthropogenic development. Their results show that landscape variables and host gene flow explained significant amounts of variation in virus spread in a region bounded by urban development but did not in a more wild landscape, demonstrating how anthropogenic landscapes can alter pathogen spread.