Mineral waters from Spring 1 and Spring 1 bis from Slănic Moldova - molecular mechanisms responsible for triggering the prophylactic and therapeutic effects

In this study, we investigated mineral water's therapeutic and prophylactic effects from springs 1 and 1 bis in Slanic Moldova, focusing on identifying the molecular mechanisms responsible for these effects. We collected water samples from these springs and analyzed their chemical composition using various analytical techniques. In addition, we tested the effects of water on cell viability using primary fibroblasts in culture and performed MTT assays to assess the metabolic activity of the cells. Our results indicate that water from both springs has beneficial properties on cells, including improving cell viability and stimulating metabolic activity. This suggests that the mineral water from springs 1 and 1 bis could have therapeutic and prophylactic potential due to its unique chemical composition. Our study could contribute to developing new mineral water-based therapies for various health conditions.