Biofilm-inspired adhesive and antibacterial hydrogel with tough tissue integration performance for sealing hemostasis and wound healing

Uncontrolled bleeding and infection can cause significant increases in mortalities. Hydrogel sealants have attracted extensive attention for their ability to control bleeding. However, because interfacial water is a formidable barrier to strong surface bonding, a challenge remains in finding a product that offers robust tissue adhesion combined with anti-infection properties. Inspired by the strong adhesive mechanism of biofilm and mussels, we report a novel dual bionic adhesive hydrogel (DBAH) based on chitosan grafted with methacrylate (CS-MA), dopamine (DA), and N-hydroxymethyl acrylamide (NMA) via a facile radical polymerization process. CS-MA and DA were simultaneously included in the adhesive polymer for imitating the two key adhesive components: polysaccharide intercellular adhesin (PIA) of staphylococci biofilm and 3,4-dihydroxy-l-phenylalanine (Dopa) of mussel foot protein, respectively. DBAH presented strong adhesion at 34 kPa even upon three cycles of full immersion in water and was able to withstand up to 168 mm Hg blood pressure, which is significantly higher than the 60–160 mm Hg measured in most clinical settings. Most importantly, these hydrogels presented outstanding hemostatic capability under wet and dynamic in vivo movements while displaying excellent antibacterial properties and biocompatibility. Therefore, DBAH represents a promising class of biomaterials for high-efficiency hemostasis and wound healing. [Display omitted] •We put forward a novel method inspired form staphylococcal biofilm for fabricating hydrogel hemostasis material. To the best of our knowledge, this is the first report of designing staphylococcal biofilm inspired adhesive hydrogel for in vivo sealing hemostasis.•Unlike the traditional acrylamide (AM) based hydrogels, the as-prepared DBAH were purified before further measurements and application by repetitive cycles of deionized water and ethyl alcohol to prevent the potential cytotoxicity of unreacted NMA monomers.•The as-prepared DBAH can intimately integrate with biological tissue and exhibit superior capacity of in vivo hemostatic by sealing the bleeding wound even in wet and dynamic rabbit's heart.