GRP78 protein metabolism in obese and diabetic rats: a study of its role in metabolic disorders

This study aimed to compare and analyze the expression and significance of the GRP78 protein in cochlear cell injury induced by a high glucose and high-fat diet in obese and diabetic rats. Male SD rats were randomly divided into two groups: normal (NC) and high-fat (HF) groups. The NC group was fed...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Diabetology and metabolic syndrome 2024-01, Vol.16 (1), p.16-16, Article 16
Hauptverfasser: Xi, Kai, Li, Hua-Ping, Wang, Yue-Hui, Li, Yang-Yang, Wang, Lei, Zhang, Miao-Miao, Zhang, Xi, Xing, Bing-Wen
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:This study aimed to compare and analyze the expression and significance of the GRP78 protein in cochlear cell injury induced by a high glucose and high-fat diet in obese and diabetic rats. Male SD rats were randomly divided into two groups: normal (NC) and high-fat (HF) groups. The NC group was fed a standard diet for eight weeks, while the HF group received a high-glucose, high-fat diet. The HF group was further categorized into the obesity group (OB group) and the type II diabetes mellitus group (T2DM group). To induce a type II diabetes mellitus (T2DM) model, the T2DM group received an intraperitoneal injection of a small dose of STZ (45 mg/kg). After four weeks on the original diet, body weight, blood glucose, blood lipid levels, and auditory brainstem response (ABR) thresholds were measured. The cochlea was dissected, and its morphology was observed using HE staining. Immunohistochemistry and western blotting were utilized to examine the expression level of the GRP78 protein in the cochlea. (1) The ABR threshold demonstrated a statistically significant difference between the T2DM group and the OB group (P 
ISSN:1758-5996
1758-5996
DOI:10.1186/s13098-024-01255-6