P18 Proteomic analysis of cutaneous lupus erythematosus (CLE) dermal infiltrates and control skin reveals differentially upregulated and downregulated pathways

BackgroundMany investigators have explored pathways upregulated in SLE and CLE. Few reports studied key downregulated pathways and mediators. Using unbiased proteomic technique, we have previously identified IL-16 as a key cytokine in cutaneous lupus erythematosus (CLE), and findings were verified lupus nephritis.1 2 In this analysis we analysed the downregulated pathways in the same proteomic database.ObjectivesTo systematically identify patterns of protein expression in dermal infiltrates of skin lesions of patients with CLE in comparison to control skin, and explore what pathways might be downregulated or non-functional.MethodsSkin biopsies from 6 CLE patients and 6 controls were investigated as described before.2 Inflammatory infiltrates and control dermis were laser capture micro-dissected and run on nano-LC tandem mass spectrometry.Data was analysed by String and Qiagen Ingenuity pathway analysis (IPA). P-values