Genetic confirmation for morphological identification of Stilesia globipunctata in camel in Iraq

Camels (Camelus dromedaries), similar to other ruminants, are infected by the Anoplocephalidae family belonging to the class cestode causing mild to severe illness. This study utilized a conventional PCR assay to confirm that Stilesia spp. is morphologically identified. Slaughtered camel intestines from the Al-Najif abattoir in the central part of Iraq were morphologically examined for Stilesia spp. Applied a PCR and genetic analysis for twenty adult worms. The presence of Stilesia spp. adult worms were initially identified through morphological characterization. The scolex and strobili were found in the intestine lumen and its nodules. All the worms’ specimens were identified as a Stilesia genus, and PCR amplified their partial DNA fragment on the location of the ITS2, 5.8S rDNA gene. Camels infected with Stilesia spp. found in eviscerated camel carcasses in south Iraq, and twenty isolates of Stilesia globipunctata molecular data have been recorded an accession numbers OM221663- OM221682 in the NCBI.