ImmunoFISH for Mice and Baboons Frozen Sections

This protocol is optimized for immunoFISH staining of OCT section of mouse tissues. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) (Le et al., 2010) and FISH against telomeric DNA.

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Bio-protocol 2013-12, Vol.3 (24)
Hauptverfasser: Rossiello, Francesca, Fumagalli, Marzia, di Fagagna, Fabrizio
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:This protocol is optimized for immunoFISH staining of OCT section of mouse tissues. It combines immunofluorescence for DNA damage response factors (e.g. 53BP1) (Le et al., 2010) and FISH against telomeric DNA.
ISSN:2331-8325
2331-8325
DOI:10.21769/BioProtoc.1000