Monitoring reactivation of latent HIV by label-free gradient light interference microscopy

Human immunodeficiency virus (HIV) can infect cells and take a quiescent and nonexpressive state called latency. In this study, we report insights provided by label-free, gradient light interference microscopy (GLIM) about the changes in dry mass, diameter, and dry mass density associated with infec...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:iScience 2021-08, Vol.24 (8), p.102940-102940, Article 102940
Hauptverfasser: Goswami, Neha, Lu, Yiyang, Kandel, Mikhail E., Fanous, Michael J., Bohn-Wippert, Kathrin, Tevonian, Erin N., Dar, Roy D., Popescu, Gabriel
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Human immunodeficiency virus (HIV) can infect cells and take a quiescent and nonexpressive state called latency. In this study, we report insights provided by label-free, gradient light interference microscopy (GLIM) about the changes in dry mass, diameter, and dry mass density associated with infected cells that occur upon reactivation. We discovered that the mean cell dry mass and mean diameter of latently infected cells treated with reactivating drug, TNF-α, are higher for latent cells that reactivate than those of the cells that did not reactivate. Cells with mean dry mass and diameter less than approximately 10 pg and 8 μm, respectively, remain exclusively in the latent state. Also, cells with mean dry mass greater than approximately 28-30 pg and mean diameter greater than 11–12 μm have a higher probability of reactivating. This study is significant as it presents a new label-free approach to quantify latent reactivation of a virus in single cells. [Display omitted] •GLIM imaging reveals differences between latent and reactivated HIV in JLat cells•Cells with reactivated HIV have higher dry mass and diameter Optics; Immunology; Virology
ISSN:2589-0042
2589-0042
DOI:10.1016/j.isci.2021.102940