Oxidized humic acids from the soil of heat power plant

Humic acids isolated from the soil of heat power plant (HA-E) contaminated with oil were analyzed by the Fourier Transform Infrared spectra (FTIR). In comparison with humic acids standard (HA-S) luck of intense broad band of stretching vibrations of hydrogen-bonded hydroxyl groups (3600-3200 cm-1) is evident. HA-E have a pick at 1649 cm-1 which could belong to carbonyl groups. HA-E are heavily oxidized and among isolated microorganisms Achromobacter denitrificans may be responsible for such intensive oxidation of HA-E. To phylogenetically diverse nitrate-reducing microorganisms that have the capacity to utilize reduced HA as electron donors in soils, we can ad A. denitrificans.