A survey of piroplasms in white-tailed deer (Odocoileus virginianus) in the southeastern United States to determine their possible role as Theileria orientalis hosts

In 2017, clinical disease and mortality in cattle associated with Theileria orientalis Ikeda was reported in Virginia, U.S. The exotic tick, Haemaphysalis longicornis, is a competent vector for this species. White-tailed deer (Odocoileus virginianus) are commonly infested with H. longicornis in the eastern U.S. and are also infected with several genotypes of piroplasms such as a Theileria sp. (often called Theileria cervi-like), Babesia odocoilei, and Babesia sp. H10. However, it is currently unknown if deer are susceptible to T. orientalis and can act as potential hosts. In this study, we tested 552 white-tailed deer samples from the southeastern U.S. to determine the presence of T. orientalis. We used a PCR-RFLP to test 293 (53%) of these samples to distinguish between piroplasm genera. A total of 189 white-tailed deer were positive with Theileria, 47 were positive with Babesia, and 57 did not amplify. Because this assay does not determine species, we sequenced 30 random samples targeting a fragment of the 18S rRNA gene. Although a high diversity of Theileria and Babesia spp. were detected, none were T. orientalis. All 552 samples were then screened with a T. orientalis specific real-time PCR protocol, but none were positive for T. orientalis. Our data suggests that white-tailed deer are commonly infected with piroplasm species but not T. orientalis. [Display omitted] •Piroplasm parasites of 552 white-tailed deer were surveyed in the Eastern U.S.•A high prevalence and diversity of Theileria and Babesia species were observed.•No infections with exotic Theileria orientalis Ikeda was detected in white-tailed deer.