Long-term Recording of LTP in Cultured Hippocampal Slices

Long-term potentiation (LTP) was elicited by high frequency stimulation in hippocampal slices cultured on multi-electrode arrays. LTP lasting more than 1 h was recorded in 75% of slices, and a significant number of slices exhibited a non-decaying LTP that lasted more than 48 h. LTP induction was com...

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Veröffentlicht in:	Neural Plasticity 2002, Vol.2002 (4), p.249-254
Hauptverfasser:	Shimono, Ken, Baudry, Michel, Ho, Lam, Taketani, Makoto, Lynch, Gary
Format:	Artikel
Sprache:	eng
Schlagworte:	2-Amino-5-phosphonovalerate - pharmacology Animals Culture Techniques - methods Electrophysiology Excitatory Amino Acid Antagonists - pharmacology Hippocampus - physiology Long-Term Potentiation Rats Rats, Sprague-Dawley Receptors, N-Methyl-D-Aspartate - physiology Time Factors
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Zusammenfassung:	Long-term potentiation (LTP) was elicited by high frequency stimulation in hippocampal slices cultured on multi-electrode arrays. LTP lasting more than 1 h was recorded in 75% of slices, and a significant number of slices exhibited a non-decaying LTP that lasted more than 48 h. LTP induction was completely and reversibly blocked by an antagonist of the NMDA receptor, APV. Our results suggest the possibility of using chronic recording in hippocampal slices cultured on multi-electrode arrays to study the mechanisms underlying LTP maintenance and stabilization.
ISSN:	0792-8483 2090-5904 1687-5443
DOI:	10.1155/NP.2002.249