FlsnRNA-seq: protoplasting-free full-length single-nucleus RNA profiling in plants

The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at...

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Veröffentlicht in:Genome Biology 2021-02, Vol.22 (1), p.66-66, Article 66
Hauptverfasser: Long, Yanping, Liu, Zhijian, Jia, Jinbu, Mo, Weipeng, Fang, Liang, Lu, Dongdong, Liu, Bo, Zhang, Hong, Chen, Wei, Zhai, Jixian
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Sprache:eng
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Zusammenfassung:The broad application of single-cell RNA profiling in plants has been hindered by the prerequisite of protoplasting that requires digesting the cell walls from different types of plant tissues. Here, we present a protoplasting-free approach, flsnRNA-seq, for large-scale full-length RNA profiling at a single-nucleus level in plants using isolated nuclei. Combined with 10x Genomics and Nanopore long-read sequencing, we validate the robustness of this approach in Arabidopsis root cells and the developing endosperm. Sequencing results demonstrate that it allows for uncovering alternative splicing and polyadenylation-related RNA isoform information at the single-cell level, which facilitates characterizing cell identities.
ISSN:1474-760X
1474-7596
1474-760X
DOI:10.1186/s13059-021-02288-0