Isolation and preliminary characterization of cyanobacteria strains from freshwaters of Greece
Cyanobacterial harmful algal blooms (or CyanoHABs) represent one of the most conspicuous waterborne microbial hazards. The characterization of the bloom communities remains problematic because the cyanobacterial taxonomy of certain genera has not yet been resolved. In this study, 29 planktic and ben...
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Veröffentlicht in: | Open life sciences 2015-01, Vol.10 (1) |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Cyanobacterial harmful algal blooms (or
CyanoHABs) represent one of the most conspicuous
waterborne microbial hazards. The characterization of
the bloom communities remains problematic because
the cyanobacterial taxonomy of certain genera has not
yet been resolved. In this study, 29 planktic and benthic
cyanobacterial strains were isolated from freshwaters
located in Greece. The strains were assigned to the genera
, and
and screened for the production of the cyanotoxins
microcystins (MCs), cylindrospermopsins (CYNs), and
saxitoxins (STXs) using molecular (PCR amplification of
seven genes implicated in cyanotoxin biosynthesis) and
immunological (ELISA) methods. This study presents,
for the first time, a cyanobacteria culture collection
from Greece, thus providing missing study material for
the understanding of bloom formation and cyanotoxin
production in the Mediterranean and for the polyphasic
characterization of important components of the
phytoplankton. The combined use of molecular and
immunochemical methods allowed the identification of
MC producing strains, but further data are needed for CYNand
STX-producing cyanobacteria. The high percentage
of MC-producing
strains in the urban Lakes
Kastoria and Pamvotis, frequently used for agriculture
irrigation, fishing and recreation, highlights the potential
risk for human health. |
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ISSN: | 2391-5412 2391-5412 |
DOI: | 10.1515/biol-2015-0006 |