Transcriptome of sessile serrated adenoma/polyps is associated with MSI‐high colorectal cancer and decreased expression of CDX2

The objective of this study was to elucidate the molecular background of sessile serrated adenoma/polyp (SSA/P) endoscopically resected with comprehensive gene expression analysis. Gene expression profiling was performed for 10 tumor‐normal pairs of SSA/P. Cluster analysis, gene set enrichment analy...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Cancer medicine (Malden, MA) MA), 2022-12, Vol.11 (24), p.5066-5078
Hauptverfasser:	Ohki, Daisuke, Yamamichi, Nobutake, Sakaguchi, Yoshiki, Takahashi, Yu, Kageyama‐Yahara, Natsuko, Yamamichi, Mitsue, Takeuchi, Chihiro, Tsuji, Yosuke, Sakai, Yasuhiro, Sakurai, Kouhei, Tomida, Shuta, Koike, Kazuhiko, Fujishiro, Mitsuhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenoma Adenoma - genetics Adenomatous polyposis coli Animals Biopsy Carcinogenesis - genetics CDX2 CDX2 protein CDX2 Transcription Factor - genetics Classification Cluster analysis Colonic Neoplasms Colonic Polyps - genetics Colorectal cancer Colorectal carcinoma Colorectal Neoplasms - genetics comprehensive gene expression analysis DNA methylation DNA microarrays Endoscopy Epithelial cells Gene expression Gene set enrichment analysis Humans Metabolism Mice Microsatellite instability microsatellite instability‐high colorectal cancer MLH1 protein MSH2 protein MSH6 protein Mutation p53 Protein Polyps Proto-Oncogene Proteins B-raf - genetics sessile serrated adenoma/polyp Software Transcriptome transcriptome analysis Transcriptomes Tumorigenesis Tumors
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The objective of this study was to elucidate the molecular background of sessile serrated adenoma/polyp (SSA/P) endoscopically resected with comprehensive gene expression analysis. Gene expression profiling was performed for 10 tumor‐normal pairs of SSA/P. Cluster analysis, gene set enrichment analysis (GSEA), and consensus molecular subtype (CMS) classification of colorectal cancer (CRC) were applied to our transcriptome analysis. Unsupervised cluster analysis showed that the gene expression profile of SSA/Ps is different from that of adjacent normal epithelial cells, even in the very early stage of tumorigenesis. According to the CMS classification, our microarray data indicated that SSA/Ps were classified as CMS1. GSEA demonstrated a strong association between SSA/P and microsatellite instability‐high (MSI‐H) CRC (p
ISSN:	2045-7634 2045-7634
DOI:	10.1002/cam4.4810