First-time serological and molecular detection of Helicobacter pylori in milk from Algerian local-breed cows

The present study was conducted to detect and identify within local cow breeds in the central region of Algeria. Two hundred (n=200) cows from three provinces of the central region of Algeria were studied, between January 2016 and September 2017. Each cow was subject to stool, milk, and blood sampling. Milk and fecal samples were used to detect and identify using bacteriology culture method. Blood and milk samples were used to detect immunoglobulin G (IgG) antibody using enzyme-linked immunosorbent assay. Polymerase chain reaction was used to confirm the abundance of in milk by detecting gene. Out of 200 sera and 200 milk samples, 12% (24) and 4% (8/200) were positive for the IgG antibody. gene was detected in the milk of 13% of cows and was confirmed in all cows presenting IgG in milk. From the present study, we concluded that the gene is an important marker for detecting in milk. Moreover, Algerian local-breed cows are a source of and could be responsible for serious zoonosis.