Intrinsic Fluorometric Reporters of Pteridine Reductase 1, a Target for Antiparasitic Agents

Intrinsic Fluorometric Reporters of Pteridine Reductase 1, a Target for Antiparasitic Agents

The intrinsic steady-state and time-resolved fluorescence of Leishmania major pteridine reductase 1, a tetrameric protein target for anti-infective agents, is investigated and deciphered in terms of the contributions from populations of the two tryptophans included in each protein monomer. Signals f...

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Veröffentlicht in:Physchem 2022-06, Vol.2 (2), p.131-144
Hauptverfasser: Ferrari, Stefania, Costi, Maria Paola, Ponterini, Glauco
Format: Artikel
Sprache:eng
Schlagworte:
Chromatography
Decomposition
Dihydrofolate reductase
Electric fields
Enzymes
fluorescence
Ligands
Parasitic diseases
protein intrinsic fluorescence
Proteins
pteridin reductase
Tropical diseases
tryptophan fluorescence
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Zusammenfassung:The intrinsic steady-state and time-resolved fluorescence of Leishmania major pteridine reductase 1, a tetrameric protein target for anti-infective agents, is investigated and deciphered in terms of the contributions from populations of the two tryptophans included in each protein monomer. Signals from these local fluorometric reporters contain molecular-level information on the conformational landscape of this protein and on its interaction with a nanomolar pteridinic inhibitor.
ISSN:2673-7167
2673-7167
DOI:10.3390/physchem2020010