The effect of diurnal light cycles on biohydrogen production in a thermosiphon photobioreactor

Hydrogen production via microbial photofermentation shows great promise as a method for sustainable hydrogen production; however, operating costs associated with photofermentative hydrogen production need to be reduced. Costs can be reduced using a passive circulation system like the thermosiphon photobioreactor, and by operating it under natural sunlight. In this study, an automated system was implemented to investigate the effect of diurnal light cycles on the hydrogen productivity and growth of Rhodopseudomonas palustris and on the operation of a thermosiphon photobioreactor, under controlled conditions. Diurnal light cycles, simulating daylight times, were found to reduce hydrogen production in the thermosiphon photobioreactor demonstrating a low maximum production rate of 0.015 mol m −3 h −1 (± 0.002 mol m −3 h −1 ) as compared to 0.180 mol m −3 h −1 (± 0.0003 mol m −3 h −1 ) under continuous illumination. Glycerol consumption as well as hydrogen yield also decreased under diurnal light cycles. Nonetheless, hydrogen production in a thermosiphon photobioreactor under outdoor conditions was demonstrated as possible avenue for further investigation. Key points The thermosiphon photobioreactor can operate under diurnal light cycles. Diurnal light cycles reduce hydrogen productivity of R. palustris . Diurnal light cycles have little to no effect on the growth of R palustris .