Histopathological changes in liver and spleen of mice infected with brucellosis

A total of 20 female mice (8 week of age)a 10 mice control and 10 deal with 10‌‌‌5cfu per animal of‌‌‌ Brucella melitensis by injection intraperitonial. Samples were collected over a 6 week period of infection. Blood collected for serological test revealed positive for animals were injected with bacteria then animal dissected, liver and spleen collected and kept in 10% formalin for histological study.Results showed Histopathological changes show congestion, granulomatous, fatty change,fibrosis in liver and increased number of lymphohistiocytic cells and increased amount of white pulp. The study concluded thatthepathological changes in liver and spleen caused by brucellamelitensis in mice are similar to those observed in humans with brucellosis