Genome-Wide Identification and Abiotic Stress Response Analysis of PP2C Gene Family in Woodland and Pineapple Strawberries

Protein phosphatase 2C (PP2C) is a negative regulator of serine/threonine residue protein phosphatase and plays an important role in abscisic acid (ABA) and abiotic-stress-mediated signaling pathways in plants. The genome complexity of woodland strawberry and pineapple strawberry is different due to the difference in chromosome ploidy. This study conducted a genome-wide investigation of the FvPP2C ( ) and FaPP2C ( ) gene family. Fifty-six genes and 228 genes were identified from the woodland strawberry and pineapple strawberry genomes, respectively. were distributed on seven chromosomes, and were distributed on 28 chromosomes. The size of the FaPP2C gene family was significantly different from that of the FvPP2C gene family, but both FaPP2Cs and FvPP2Cs were localized in the nucleus, cytoplasm, and chloroplast. Phylogenetic analysis revealed that 56 and 228 could be divided into 11 subfamilies. Collinearity analysis showed that both and had fragment duplication, and the whole genome duplication was the main cause of gene abundance in pineapple strawberry. mainly underwent purification selection, and there were both purification selection and positive selection effects in the evolution of . -acting element analysis found that the family genes of woodland and pineapple strawberries mainly contained light responsive elements, hormone responsive elements, defense and stress responsive elements, and growth and development-related elements. The results of quantitative real-time PCR (qRT-PCR) showed that the genes showed different expression patterns under ABA, salt, and drought treatment. The expression level of was upregulated after stress treatment, which may play a positive regulatory role in ABA signaling and abiotic stress response mechanisms. This study lays a foundation for further investigation on the function of the PP2C gene family.