Isolation of Legionella pneumophila by Co-culture with Local Ameba, Canada

Legionellosis was diagnosed in an immunocompromised 3-year-old girl in Canada. We traced the source of the bacterium through co-culture with an ameba collected from a hot tub in her home. We identified Legionella pneumophila serogroup 6, sequence type 185, and used whole-genome sequencing to confirm...

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Veröffentlicht in:	Emerging infectious diseases 2019-11, Vol.25 (11), p.2104-2107
Hauptverfasser:	Dey, Rafik, Mount, Harley, Ensminger, Alex W, Tyrrell, Greg J, Ward, Linda P, Ashbolt, Nicholas J
Format:	Artikel
Sprache:	eng
Schlagworte:	ameba Amoeba - microbiology Antibodies by Co-culture with Local Ameba, Canada Canada - epidemiology case report coculture Coculture Techniques Disease Outbreaks Dispatch DNA sequencing Environmental toxicology Genome, Bacterial Genomes Genomics Health hot tub Humans Isolation of Legionella pneumophila Legionella pneumophila - classification Legionella pneumophila - genetics Legionella pneumophila - isolation & purification Legionellosis Legionnaires' disease Legionnaires' Disease - epidemiology Legionnaires' Disease - microbiology Lung diseases Meropenem Phylogeny Public health Public Health Surveillance Respiratory tract diseases Whole Genome Sequencing
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Zusammenfassung:	Legionellosis was diagnosed in an immunocompromised 3-year-old girl in Canada. We traced the source of the bacterium through co-culture with an ameba collected from a hot tub in her home. We identified Legionella pneumophila serogroup 6, sequence type 185, and used whole-genome sequencing to confirm the environmental and clinical isolates were of common origin.
ISSN:	1080-6040 1080-6059
DOI:	10.3201/eid2511.190522