Real-time polymerase chain reaction for detecting SARS coronavirus, Beijing, 2003

During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay...

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Veröffentlicht in:	Emerging infectious diseases 2004-02, Vol.10 (2), p.300-316
Hauptverfasser:	Zhai, Junhui, Briese, Thomas, Dai, Erhei, Wang, Xiaoyi, pang, Xin, Du, Zongmin, Liu, Haihong, Wang, Jin, Wang, Hongxia, Guo, Zhaobiao, Chen, Zeliang, Jiang, Lingxiao, Zhou, Dongsheng, Han, Yanping, Jabado, Omar, Palacios, Gustavo, Lipkin, W Ian, Tang, Ruifu
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Viral - blood Base Sequence China China - epidemiology diagnostic Disease Outbreaks Dispatch DNA Primers - genetics DNA, Viral - genetics Feces - virology Humans PCR Polymerase Chain Reaction - methods Polymerase Chain Reaction - standards RNA, Viral - blood RNA, Viral - genetics RNA, Viral - isolation & purification SARS coronavirus SARS Virus - genetics SARS Virus - immunology SARS Virus - isolation & purification SARS-CoV Severe Acute Respiratory Syndrome - epidemiology Severe Acute Respiratory Syndrome - immunology Severe Acute Respiratory Syndrome - virology Time Factors
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Zusammenfassung:	During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3' end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.
ISSN:	1080-6040 1080-6059
DOI:	10.3201/eid1002.030799