Effect of natural selection on common bean (Phaseolus vulgaris) microsatellite alleles

The effect of natural selection on microsatellite simple sequence repeat (SSR) alleles was investigated in two distinct common bean (Phaseolus vulgaris) generations (F sub(8) and F sub(24)) derived from the cross between the P. vulgaris cultivars Carioca MG x ESAL 686. The F sub(2) segregant populat...

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Veröffentlicht in:Genetics and molecular biology 2006-01, Vol.29 (2), p.345-352
Hauptverfasser: Rodrigues, Taislene Butarello, Santos, João Bosco dos
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Sprache:eng
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Zusammenfassung:The effect of natural selection on microsatellite simple sequence repeat (SSR) alleles was investigated in two distinct common bean (Phaseolus vulgaris) generations (F sub(8) and F sub(24)) derived from the cross between the P. vulgaris cultivars Carioca MG x ESAL 686. The F sub(2) segregant population was propagated by the bulk method and 107 plants were sampled in two generations (F sub(8) and F sub(24)). Each plant generated one family which was replicated by the bulk method to F sub(8:11) and F sub(24:27) families from which DNA was extracted. Thirty pairs of microsatellite primers were polymorphic for the parents and the bulk of the F sub(24:27) families. Out of 30 loci selected by natural selection, 29 microsatellite alleles came from the Carioca MG parent and one allele came from the ESAL 686 parent. Natural selection affected all the generations and its intensity was specific for each locus and generation. Therefore all the alleles selected at each locus must be important for adaptation in a breeding program.
ISSN:1415-4757
1678-4685
1415-4757
1678-4685
DOI:10.1590/S1415-47572006000200024