Simultaneous Assay of CA 72-4, CA 19-9, CEA and CA 125 in Biological Samples Using Needle Three-Dimensional Stochastic Microsensors

Two-needle 3D stochastic microsensors based on boron- and nitrogen-decorated gra-phenes, modified with N-(2-mercapto-1H-benzo[d]imidazole-5-yl), were designed and used for the molecular recognition and quantification of CA 72-4, CA 19-9, CEA and CA 125 biomarkers in biological samples such as whole...

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Veröffentlicht in:Sensors (Basel, Switzerland) Switzerland), 2023-09, Vol.23 (19), p.8046
Hauptverfasser: Bratei, Alexandru-Adrian, Stefan-van Staden, Raluca-Ioana, Ilie-Mihai, Ruxandra-Maria, Gheorghe, Damaris-Cristina
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Sprache:eng
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Zusammenfassung:Two-needle 3D stochastic microsensors based on boron- and nitrogen-decorated gra-phenes, modified with N-(2-mercapto-1H-benzo[d]imidazole-5-yl), were designed and used for the molecular recognition and quantification of CA 72-4, CA 19-9, CEA and CA 125 biomarkers in biological samples such as whole blood, urine, saliva and tumoral tissue. The NBGr-2 sensor yielded lower limits of determination. For CEA, the LOD was 4.10 × 10−15 s−1 g−1 mL, while for CA72-4, the LOD was 4.00 × 10−11 s−1 U−1 mL. When the NBGr-1 sensor was employed, the best results were obtained for CA12-5 and CA19-9, with values of LODs of 8.37 × 10−14 s−1 U−1 mL and 2.09 × 10−13 s−1 U−1 mL, respectively. High sensitivities were obtained when both sensors were employed. Broad linear concentration ranges favored their determination from very low to higher concentrations in biological samples, ranging from 8.37 × 10−14 to 8.37 × 103 s−1 U−1 mL for CA12-5 when using the NBGr-1 sensor, and from 4.10 × 10−15 to 2.00 × 10−7 s−1 g−1 mL for CEA when using the NBGr-2 sensor. Student’s t-test showed that there was no significant difference between the results obtained utilizing the two microsensors for the screening tests, at a 99% confidence level, with the results obtained being lower than the tabulated values.
ISSN:1424-8220
1424-8220
DOI:10.3390/s23198046