Effect of Chronic Nicotine Administration on the Pro-oxidant Antioxidant Balance of Mice Serum: Role of Rosmarinic Acid

Background and Aim: In this experimental study, nicotine has been recognized to result in oxidative stress by inducing the generation of reactive oxygen species. The current research was designed to investigate the protective effect of rosmarinic acid, a radical scavenger and antioxidant, on Pro oxidant anti-oxidant balance of serum in nicotine treated mice. Materials and Methods: Thirty-six mature male NMRI mice were divided into 6groups: (two controls, two nicotine-treated [0.5 mg/kg], and two nicotine plus rosmarinic acid [10 mg/kg]) were used in this study and treated for 15 and 30days respectively. The standard protocol was used to measure pro-oxidant-antioxidant balance, superoxide dismutase, and serum Catalase. Results: As compared to control group (90±3.03315 HK), the 15-day results, in nicotine-treated group (100±5.17687 HK) there was a significant increase in the serum PAB ratio. Similarly, for samples of the day 30, there was a significant increase in the serum PAB ratio of nicotine-treated group (106±3.52136 HK) versus control group (87±1.32916 HK) (P≤0.05). SOD of 15(1.21±1.12) and 30 days (1.89±0.26) treated groups, showed significant decreased versus control groups (2.90±0.09), (2.82±0.08) respectively (P≤0.05). Catalase of 15(12.13±2.30) and 30 days (11.57±1.42) treated groups, showed significant decreased versus control groups (25.12±2.21), (24.1±1.29) respectively (P≤0.05). Conclusion: These results indicate that rosmarinic acid improves the level of antioxidant enzymes and modifies pro-oxidant-antioxidant imbalance