Understanding the effects of omega-3 fatty acid supplementation on the physical properties of brain lipid membranes

A deficiency in omega-3 fatty acids (ω3 FAs) in the brain has been correlated with cognitive impairment, learning deficiencies, and behavioral changes. In this study, we provided ω3 FAs as a supplement to spontaneously hypertensive rats (SHR+ ω3). Our focus was on examining the impact of dietary supplementation on the physicochemical properties of the brain-cell membranes. Significant increases in ω3 levels in the cerebral cortex of SHR+ ω3 were observed, leading to alterations in brain lipid membranes molecular packing, elasticity, and lipid miscibility, resulting in an augmented phase disparity. Results from synthetic lipid mixtures confirmed the disordering effect introduced by ω3 lipids, showing its consequences on the hydration levels of the monolayers and the organization of the membrane domains. These findings suggest that dietary ω3 FAs influence the organization of brain membranes, providing insight into a potential mechanism for the broad effects of dietary fat on brain health and disease. [Display omitted] •ω3 FAs alters brain lipid membranes, at molecular and nanoscale levels•ω3 FAs induce changes in molecular packing, elasticity, and lipid miscibility•ω3 FAs increase lipid phase disparity•Future research aims to unveil how ω3 levels influence cognition and behavior Membrane architecture; Neuroscience; Cell biology