A Turn-On Fluorescent Probe for Highly Selective Detection and Visualization of Hydrogen Sulfide in Fungi

Hydrogen sulfide (H S) is a significant actor in the virulence and pathogenicity of fungi. The analysis of endogenous H S in fungi benefits the prevention and treatment of pathogenic infections. Herein, a H S-activated turn-on fluorescent probe named was developed for the sensitive and selective detection of H S. Using , the ability of several oral fungi strains to produce H S was identified, which was also validated using a typical chromogenic medium. In addition, was successfully used for the visual sensing of endogenous H S in fungal cells via microscope, flow cytometry, and colony imaging, along with a specific validation with the co-incubation of H S production inhibitors in living cells. Above all, could be used for H S detection, the fluorescent imaging of fungi, and even the identification of related fungi.