Loop-mediated isothermal amplification for influenza A (H5N1) virus

We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay prod...

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Veröffentlicht in:	Emerging infectious diseases 2007-06, Vol.13 (6), p.899-901
Hauptverfasser:	Jayawardena, Shanthi, Cheung, Chung Y, Barr, Ian, Chan, Kwok H, Chen, Honglin, Guan, Yi, Peiris, J S Malik, Poon, Leo L M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Avian influenza avian influenza virus Biological assay Diagnosis Diagnostic virology Dispatch DNA, Viral - classification DNA, Viral - genetics H5N1 Humans Influenza A Virus, H5N1 Subtype - classification Influenza A Virus, H5N1 Subtype - genetics Influenza in Birds - diagnosis Influenza in Birds - genetics Influenza in Birds - virology Influenza, Human - diagnosis Influenza, Human - genetics Influenza, Human - virology loop-mediated isothermal amplification molecular detection Molecular Diagnostic Techniques - methods Molecular Sequence Data Poultry - virology Virology - methods
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Zusammenfassung:	We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
ISSN:	1080-6040 1080-6059
DOI:	10.3201/eid1306.061572