Characterization of silver sulfide nanoparticles from actinobacterial strain (M10A62) and its toxicity against lepidopteran and dipterans insect species

In this research, cell-free extracts from magnesite mine-isolated actinobacterial strain (M10A62) were used to produce silver sulfide nanoparticles (Ag SNPs). JX905302, actinobacteria capable of producing Ag SNPs, was used to synthesize Ag NPs. The UV–vis range was used to confirm the biosynthesized Ag NPs; Fourier transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), transmission electron microscopy (TEM), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDAX), and dynamic light scattering analysis were employed to characterize them further. Surface resonance plasma (SRP) for Ag SNPs was obtained at 355 nm using UV–visible spectroscopy; FT-IR detected bimolecular and eventually microbial-reduced Ag SNPs from culture extract. Furthermore, AFM and TEM analysis confirms that the synthesized Ag SNPs were spherical in shape. Dynamic light scattering revealed a negatively charged Ag NPs surface with a diameter of 10 nm. The XRD spectrum showed the crystalline nature of the obtained particles. EDAX revealed a pure crystalline nature, and a significant silver particle signal confirms the presence of metallic silver and sulfide nanoparticles together with the signals of Cu and C atoms. After 40 and 48 h of treatment at 150–200 µg·ml , Ag SNPs produced the highest mortality in , , and larvae. Hence, the biosynthesized Ag SNPs may be useful for potential pest control in integrated pest management and vector control program as a safer, cost-effective, selective, and environmentally friendly approaches.