Prevalence, and virulence determination of Listeria monocytogenes strains isolated from clinical and non-clinical samples by multiplex polymerase chain reaction

This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. L. monocytogenes was...

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Veröffentlicht in:	Revista da Sociedade Brasileira de Medicina Tropical 2016-09, Vol.49 (5), p.624-627
Hauptverfasser:	Pournajaf, Abazar, Rajabnia, Ramazan, Sedighi, Mansour, Kassani, Aziz, Moqarabzadeh, Vahid, Lotfollahi, Lida, Ardebilli, Abdollah, Emadi, Behzad, Irajian, Gholamreza
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Animals, Domestic - microbiology Dairy Products - microbiology Female Food Microbiology Genes Genotype Humans Iran Listeria monocytogenes Listeria monocytogenes - genetics Listeria monocytogenes - isolation & purification Listeria monocytogenes - pathogenicity Meat - microbiology Multiplex Polymerase Chain Reaction Multiplex-PCR Pathogenesis Polymerase chain reaction Prevalence Serotyping Studies TROPICAL MEDICINE Virulence - genetics Virulence genes
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Zusammenfassung:	This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, and inlJ were detected in 100%, 76.26%, and 71% isolates, respectively. This study validated MPCR in the analysis and rapid detection of L. monocytogenes. The role of the genes in pathogenesis of the strains can also be affirmed.
ISSN:	0037-8682 1678-9849 1678-9849 0037-8682
DOI:	10.1590/0037-8682-0403-2015