Plasmonic Nanostructure Functionalization for Surface-Enhanced Fluorescence Bio-Detection

Plasmonic nanostructures represent a suitable platform for the detection of biomolecule interactions. Their surface functionalization can be performed through different strategies. Optimal thickness, homogeneity, and hydrophilicity of the functional layer can play a crucial role in defining the sensing capabilities required to perform bioassays. In this framework, a combination of tetraethylorthosilicate (TEOS) and a commercial polymer (MCP) was evaluated to improve these features. In our more recent studies, we focused on plasmon-enhanced fluorescence for the detection of a microbial-derived synthetic oligonucleotide. An effective improvement of the fluorescence signal was detected for the combined TEOS and MCP coating.