Reduced Expression of PLCXD3 Associates With Disruption of Glucose Sensing and Insulin Signaling in Pancreatic β-Cells
Previous work has shown that reduced expression of PLCXD3 , a member of the phosphoinositide-specific phospholipases (PI-PLC) family, impaired insulin secretion with an unclear mechanism. In the current study, we aim to investigate the mechanism underlying this effect using human islets and rat INS-...
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Veröffentlicht in: | Frontiers in endocrinology (Lausanne) 2019-11, Vol.10, p.735-735 |
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Sprache: | eng |
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Zusammenfassung: | Previous work has shown that reduced expression of
PLCXD3
, a member of the phosphoinositide-specific phospholipases (PI-PLC) family, impaired insulin secretion with an unclear mechanism. In the current study, we aim to investigate the mechanism underlying this effect using human islets and rat INS-1 (832/13) cells. Microarray and RNA sequencing data showed that
PLCXD3
is among the highly expressed PI-PLCs in human islets and INS-1 (832/13) cells. Expression of
PLCXD3
was reduced in human diabetic islets, correlated positively with
Insulin
and
GLP1R
expression and inversely with the donor's body mass index (BMI) and glycated hemoglobin (HbA
1c
). Expression silencing of
PLCXD3
in INS-1 (832/13) cells was found to reduce glucose-stimulated insulin secretion (GSIS) and insulin content. In addition, the expression of
Insulin, NEUROD1, GLUT2, GCK, INSR, IRS2
, and
AKT
was downregulated. Cell viability and apoptosis rate were unaffected. In conclusion, our data suggest that low expression of
PLCXD3
in pancreatic β-cells associates with downregulation of the key insulin signaling and insulin biosynthesis genes as well as reduction in glucose sensing. |
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ISSN: | 1664-2392 1664-2392 |
DOI: | 10.3389/fendo.2019.00735 |