Detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction using repetitive 529 bp gene in feces of pet cats ( Felis catus ) in Yogyakarta, Indonesia

The aim of this research was to determine the copro-prevalence of using polymerase chain reaction (PCR) with repetitive 529 bp gene and to construct the phylogenetic tree of oocyst from pet cats in Yogyakarta. 9 of 132 pet cat samples which serologically positive for were used in this research. To determine the copro-prevalence of in pet cat, 10 g of feces samples taken from practitioners and household cats in Yogyakarta were used in the PCR method utilizing repetitive 529 bp gene sequences. The result shows that copro-prevalence by PCR using repetitive 529 bp gene was 33.3% (3/9). The phylogenetic tree of grouped into two clades, which clade 1 consists of isolates collected from pet cats in Yogyakarta Indonesia and isolates from China and in clade 2 consist of the isolates from India. Copro-prevalence of in pet cats in Yogyakarta by means of PCR using repetitive 529 bp gene is around 33.3%.