Acute toxicity and anti-inflammatory activity of bis-thiourea derivatives

In the current work, bis-thiourea derivatives have been synthesized through condensation reaction between isothiocynates and diamines in dry acetone to form (1,2-bis( -benzoylthioureido) benzene), (1,3-bis( -benzoylthioureido) benzene), and (1,4-bis( -benzoylthioureido) benzene). The structures of new synthesized derivatives were confirmed through melting point and spectroscopic technique such as HNMR only. The synthesized compounds were assessed for acute toxicity test and are proved free of toxicity. The derivatives were further tested as anti-inflammatory agents by lipoxygenase enzyme inhibition studies, molecular docking, and carrageenan-induced paw edema assay, and histamine-induced edema test. The overall observations presented that compounds and possess promising anti-inflammatory potential, while compound SK2 is found to be a good anti-inflammatory agent.