Bioinformatics Approaches for Structural and Functional Annotation of an Uncharacterized Protein of Helicobacter pylori
Helicobacter pylori (H. pylori) is a Gram-negative, slow-growing bacterium, microaerophilic, and a unique gastric pathogen that causes chronic inflammation in the gastric mucosa with the possibility of developing gastric cancer; one-third of its proteins are still uncharacterized. In this study, com...
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Veröffentlicht in: | Engineering proceedings 2023-05, Vol.37 (1), p.61 |
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Sprache: | eng |
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Zusammenfassung: | Helicobacter pylori (H. pylori) is a Gram-negative, slow-growing bacterium, microaerophilic, and a unique gastric pathogen that causes chronic inflammation in the gastric mucosa with the possibility of developing gastric cancer; one-third of its proteins are still uncharacterized. In this study, computational analysis was performed on the structural, functional, and epitopic characteristics of a hypothetical protein (HP) from H. pylori named HPF63-1454. The model prediction and primary, secondary, and three-dimensional structures of the chosen HP were built. The newly created model appeared to have high quality once refinement and structure validation had been completed. The anticipated tertiary structure was assessed using the Swiss Model assessment. The best materials were chosen using structural analyses and considering data from the Z scores, Swiss Interactive Workplace, and Ramachandran plot statistics. This investigation aimed to determine the importance of the H. pylori protein HPF63-1454. Therefore, this research increased our understanding of pathophysiology and allowed us to target the protein complex specifically. H. pylori infections are now treated with readily available antibiotics and acid-suppressing medications, but this species has established itself as a hardy pest. Long-term and taxing antibiotic therapy for H. pylori infection is increasingly beginning to fail, necessitating novel and creative therapeutic modalities. |
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ISSN: | 2673-4591 |
DOI: | 10.3390/ECP2023-14671 |