Liver pathological changes and hepatocyte acquisition method of model mice with non-alcoholic fatty liver disease

Objective To detect the pathological changes in the liver of mice with non-alcoholic fatty liver disease (NAFLD) and explore an experimental method to obtain a large number of primary hepatocytes with high activity and purity from the mice with NAFLD. Methods A total of 60 mice were randomly divided into control group (chow diet, CD) and high-fat group (high-fat diet, HFD) and had been fed separately for 20 weeks. The body weight of the mice was regularly measured and the pathological characteristics of the liver tissue were observed by hematoxylin-eosin (HE) staining and Sirius red staining. After 15 weeks of high-fat diet, 6-8 mL collagenase Ⅳ were added for digestion to obtain mixed hepatocytes, which were spread on 40% Percoll separation solution and subjected to density gradient centrifugation to obtain purified hepatocytes. The viability of the cells was determined by trypan blue staining, and the morphology was observed by microscope. Results With the prolongation of modeling time, the body weight of m