Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes

Comparison of Recombinant MVA Selection Methods Based on F13L, D4R and K1L Genes

Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly impr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Viruses 2022-03, Vol.14 (3), p.528
Hauptverfasser: Antoshkina, Irina V, Glazkova, Dina V, Urusov, Felix A, Bogoslovskaya, Elena V, Shipulin, German A
Format: Artikel
Sprache:eng
Schlagworte:
Cell Line
Cloning
Genes
Genes, Viral
Genetic Vectors - genetics
Genomes
host-range selection
Immunogenicity
Infections
Kinases
MVA
Phenotypes
Plasmids
recombinant MVA
Vaccines
Vaccinia
vaccinia virus
Vaccinia virus - genetics
Vectors (Biology)
Viruses
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Modified vaccinia Ankara (MVA) is a promising vaccine vector due to its highly attenuated phenotype and good immunogenicity. However, obtaining a new recombinant MVA remains a tedious and laborious procedure involving many rounds of plaque purification. Recombinant MVA generation can be greatly improved and facilitated by different selection techniques. Here, we describe a comparison between techniques based on K1L, F13L and D4R genes.
ISSN:1999-4915
1999-4915
DOI:10.3390/v14030528