Effects of polysaccharide-based silver and selenium nanoparticles on growth performance, biochemical parameters, and immune response of Cyprinus carpio

•Comparative effects of polysaccharide-based AMLP-AgNPs and AMLP-SeNPs on Cyprinus carpio were investigated.•Fish that received dietary AMLP-SeNPs showed enhanced growth performance,improved immune systems , antioxidant defense systems and normalize blood biochemical parameters.•Dietary AMLP-SeNPs and AMLP-AgNPs showed a higher percentage of survivability after A. hydrophila infection.•Total A. hydrophila count in blood and muscles was found to be lower in fish fed with dietary AMLP-SeNPs followed by AMLP-AgNPs.•These findings suggested that applying nanotechnology to aquaculture could offer up new opportunities, such as reducing feed nutrient losses, increasing faster growth. Avicennia marina mangrove leaves polysaccharide (AMLP) was used for the synthesis of polysaccharide-based selenium (AMLP-SeNPs) and silver nanoparticles (AMLP-AgNPs). The synthesized nanoparticles were further characterized by UV-Vis, DLS, FT-IR, X-ray diffraction, and HR-TEM analysis. A 60-day (8 weeks) feeding trial experiment was conducted to investigate the effects of AMLP, AMLP-SeNPs, and AMLP-AgNPs dietary supplementation on growth performance parameters, blood parameters, immunological and enzymatic profiles in Cyprinus carpio. The characterization results of AMLP-SeNPs and AMLP-AgNPs confirmed the formation of well-stabilized spherical nanoparticles with a mean particle size of 37.25 and 72.40 nm, respectively having a crystalline structure. The feeding experiment results demonstrated that 2 mg/kg of AMLP-SeNPs followed by 0.2 mg/kg of AMLP-AgNPs showed significantly (p ˂ 0.05) higher final weight, weight gain (WG), specific growth rate (SGR%), protein and lipid efficiency, and lower food conversion ratio as compared to other groups. The catalase, superoxidase dismutase, and glutathione peroxidase activity were significantly (p ˂ 0.05) higher in the group fed 2 mg/kg supplemented AMLP-SeNPs. Total protein and globulin contents were significantly (p ˂ 0.05) higher and albumin concentration was significantly lower in fish that received 2 mg/kg of AMLP- SeNPs as compared to control. A significant increase in serum HDL and decrease in LDL and MDA concentrations were observed in the group supplemented with 2 mg/kg of nano selenium. The body's crude lipid, protein, moisture, and ash were not significantly different from the control. The AMLP-SeNPs showed significantly (p ˂ 0.05) lower aspartate aminotransferase (AST), alanine aminotransferase (ALT), and higher alkaline phosphatase