Impact of rumen-protected glucose on performance, milk composition, and selected blood metabolites of early lactating Holstein Friesian cows

High-producing dairy cows often face calving stress and reduced feed intake during the transition period, leading to body fat mobilization to meet production demands. Supplementing rations with energy-dense sources like rumen-protected glucose (RPG) may enhance production performance in early lactation. This study evaluated the effects of RPG supplementation on feed intake, body condition score (BCS), production performance, and blood metabolites in 32 early-lactation Holstein Friesian cows (6 ± 1 DIM; milk yield: 30 ± 5 kg/day; body weight: 550 ± 50 kg; BCS: 3.00 ± 0.25). Cows were assigned to four groups ( = 8/group) and fed a basal diet (Control) or supplemented with 150 g (S-150), 300 g (S-300), or 450 g (S-450) of RPG for a 42-day trial after 2 weeks of adaptation. Results showed significant improvements ( 0.05), blood glucose levels rose, and non-esterified fatty acids and -hydroxybutyric acid concentrations decreased (