Quercetin ameliorates hyperuricemic nephropathy by repressing uric acid synthesis and reabsorption in mice and cells
Hyperuricemia as one of the key factors for gout, and quercetin could reduce the uric acid (UA) content. However, the mechanism of quercetin reduces UA content is still uncovered. Herein, we found quercetin can reduce UA content, induce antioxidant enzyme activity, inhibit the enzyme activity of xan...
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Veröffentlicht in: | eFood (Amsterdam) 2024-04, Vol.5 (2), p.n/a |
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Sprache: | eng |
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Zusammenfassung: | Hyperuricemia as one of the key factors for gout, and quercetin could reduce the uric acid (UA) content. However, the mechanism of quercetin reduces UA content is still uncovered. Herein, we found quercetin can reduce UA content, induce antioxidant enzyme activity, inhibit the enzyme activity of xanthine oxidase (XOD) in hyperuricemia mice. Moreover, the UA reabsorption was also significantly reduced in the kidney and the kidney inflammation was alleviated by quercetin in hyperuricemia mice. Meanwhile, the protein level of ATP‐binding cassette super‐family G member 2 (ABCG2) was markedly induced and the protein levels of urate transporter 1 (URAT1) and glucose transporter 9 (GLUT9) were significantly repressed by quercetin in hyperuricemia mice and HK‐2 cells and by quercetin‐3‐O‐glucuronide in HK‐2 cells. Additionally, the docking mode indicated that quercetin is stable bound to the active site of XOD. Overall, the data reveal that quercetin alleviates hyperuricemia by repressing the activity of XOD, reducing the UA absorption via enhancing ABCG2 expression, and repressing URAT1 and GLUT9 expression.
Quercetin significantly reduces UA content by alleviating kidney symptom, improving antioxidant ability, inhibiting XOD activity by binding its active sites, and repressing UA reabsorption and inducing UA excretion via inhibiting URAT1 and GLUT9 proteins expression and inducing ABCG2 protein expression in hyperuricemia mice. Moreover, quercetin and its main metabolism quercetin‐3‐O‐glucoside can also inhibit UA reabsorption and enhance UA excretion via repressing URAT1 and GLUT9 and inducing ABCG2 protein expression in cells. |
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ISSN: | 2666-3066 2666-3066 |
DOI: | 10.1002/efd2.139 |