Neutrophil pyroptosis regulates corneal wound healing and post‐injury neovascularisation
Rationale The cornea is a unique structure that maintains its clarity by remaining avascular. Corneal injuries can lead to neovascularisation (CNV) and fibrosis and are the third most common cause of blindness worldwide. Objective Corneal injuries induce an immune cell infiltration to initiate repar...
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Veröffentlicht in: | Clinical and Translational Medicine 2024-11, Vol.14 (11), p.e1762-n/a |
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Sprache: | eng |
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Zusammenfassung: | Rationale
The cornea is a unique structure that maintains its clarity by remaining avascular. Corneal injuries can lead to neovascularisation (CNV) and fibrosis and are the third most common cause of blindness worldwide.
Objective
Corneal injuries induce an immune cell infiltration to initiate reparative processes. However, inflammation caused by sustained immune cell infiltration is known to be detrimental and can delay the healing process. This study was designed to understand the potential role of neutrophil and epithelial cell crosstalk in post‐injury CNV.
Methods and results
Western blotting and immunostaining assays demonstrated that neutrophils infiltrated corneas and underwent pyroptosis following acute alkali injury. In vivo studies showed that genetic ablation of Gasdermin D (GsdmD), a key effector of pyroptosis, enhanced corneal re‐epithelialisation and suppressed post‐injury CNV. In vitro co‐culture experiments revealed that interleukin‐1β (IL‐1β) was released from pyroptotic neutrophils which suppressed migration of murine corneal epithelial cells. Real‐time RT‐PCR and immunostaining assays identified two factors, Wnt5a and soluble fms‐like tyrosine kinase‐1 (sflt‐1), highly expressed in newly healed epithelial cells. sflt‐1 is known to promote corneal avascularity. Bone marrow transplantation, antibody mediated neutrophil depletion, and pharmacological inhibition of pyroptosis promoted corneal wound healing and inhibited CNV in an in vivo murine corneal injury model.
Conclusion
Taken together, our study reveals the importance of neutrophil/epithelium crosstalk and neutrophil pyroptosis in response to corneal injuries. Inhibition of neutrophil pyroptosis may serve as a potential treatment to promote corneal healing without CNV.
Key points
Neutrophil pyroptosis delays re‐epithelialization after corneal injury
Compromised re‐epithelialization promotes corneal neovascularization after injury
Inhibition of post‐injury pyroptosis could be an effective therapy to promote corneal wound healing.
• Neutrophil pyroptosis delays re‐epithelialisation after corneal injury;
• Compromised re‐epithelialisation promotes corneal neovascularisation after injury;
• Inhibition of post‐injury pyroptosis could be an effective therapy to promote corneal wound healing. |
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ISSN: | 2001-1326 2001-1326 |
DOI: | 10.1002/ctm2.1762 |