Methods and Applications of Three-Color Confocal Imaging

A simple method for constructing two and three-color merged images from grayscale confocal fluorescence images using Adobe Photoshop is outlined. Various computer methods for manipulating and displaying the images are discussed in light of several recent biomedical applications of multi-label confoc...

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Veröffentlicht in:	BioTechniques 1997-01, Vol.22 (1), p.120-126
Hauptverfasser:	Paddock, Stephen W, Hazen, Eric J, DeVries, Peter J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Drosophila - embryology Embryo, Nonmammalian - cytology Fluorescence Image Processing, Computer-Assisted - methods Investigative techniques, diagnostic techniques (general aspects) Medical sciences Microscopy, Confocal - methods Miscellaneous. Technology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Software Videotape Recording
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Beschreibung
Zusammenfassung:	A simple method for constructing two and three-color merged images from grayscale confocal fluorescence images using Adobe Photoshop is outlined. Various computer methods for manipulating and displaying the images are discussed in light of several recent biomedical applications of multi-label confocal microscopy.
ISSN:	0736-6205 1940-9818
DOI:	10.2144/97221st04