Differential gene expression analysis of common target genes for the detection of SARS-CoV-2 using real time-PCR

COVID-19 currently is the main cause of the severe acute respiratory disease and fatal outcomes in human beings worldwide. Several genes are used as targets for the detection of SARS-CoV-2, including the RDRP, N, and E genes. The present study aimed to determine the RDRP, N, and E genes expressions...

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Veröffentlicht in:AMB Express 2022-09, Vol.12 (1), p.112-112, Article 112
Hauptverfasser: Valadan, Reza, Golchin, Soheila, Alizadeh-Navaei, Reza, Haghshenas, Mohammadreza, Zargari, Mehryar, Mousavi, Tahoora, Ghamati, Mohammad
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Sprache:eng
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Zusammenfassung:COVID-19 currently is the main cause of the severe acute respiratory disease and fatal outcomes in human beings worldwide. Several genes are used as targets for the detection of SARS-CoV-2, including the RDRP, N, and E genes. The present study aimed to determine the RDRP, N, and E genes expressions of SARS-CoV- 2 in clinical samples. For this purpose, 100 SARS-CoV-2 positive samples were collected from diagnostic laboratories of Mazandaran province, Iran. After RNA extraction, the real-time reverse transcription PCR (real-time RT-PCR) assay was performed for differential gene expressions’ analysis of N, E, and RDRP. The threshold cycle (Ct) values for N, RDRP, and E targets of 100 clinical samples for identifying SARS-CoV-2 were then evaluated using quantitative real-time PCR (qRT-PCR). This result suggests N gene as a potential target for the detection of the SARS-CoV‐2, since it was observed to be highly expressed in the nasopharyngeal or oropharynges of COVID-19 patients (P 
ISSN:2191-0855
2191-0855
DOI:10.1186/s13568-022-01454-2