Pigs Immunized with the Virus-like Particle Vaccine Are Protected against the Hepatitis E-3 Virus

In this study, we generated the HEV virus-like particle (VLP) vaccine expressing 239 amino acids (367–605 aa) of the HEV-3 ORF2 using the baculovirus expression system. The HEV-3-239-VLP vaccine efficacy was evaluated by dividing 12 pathogen-free pigs into four groups: negative control, positive con...

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Veröffentlicht in:Vaccines (Basel) 2021-11, Vol.9 (11), p.1265
Hauptverfasser: Go, Hyeon-Jeong, Park, Byung-Joo, Ahn, Hee-Seop, Kim, Dong-Hwi, Kim, Da-Yoon, Kim, Jae-Hyeong, Lee, Joong-Bok, Park, Seung-Yong, Song, Chang-Seon, Lee, Sang-Won, Choi, Yang-Kyu, Choi, In-Soo
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Sprache:eng
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Zusammenfassung:In this study, we generated the HEV virus-like particle (VLP) vaccine expressing 239 amino acids (367–605 aa) of the HEV-3 ORF2 using the baculovirus expression system. The HEV-3-239-VLP vaccine efficacy was evaluated by dividing 12 pathogen-free pigs into four groups: negative control, positive control, 100 μg VLP-, and 200 μg VLP-vaccinated groups for 10 weeks. The pigs in either of the vaccinated groups were administered the corresponding first and booster doses on weeks 0 and 2. At week 4, the positive control and two vaccinated groups were challenged with 106 HEV-3 genomic equivalent copies; viremia and fecal shedding of the virus were identified in pigs in the positive control and 100 μg VLP-vaccinated pigs showed transient viremia and fecal viral shedding. However, no viruses were detected in the serum or fecal samples of the 200 μg VLP-vaccinated pigs. The 100 and 200 μg VLP-vaccinated pigs had significantly higher (p < 0.01) anti-HEV antibodies than the negative control pigs from weeks 6–10 with normal levels of liver enzymes. The 200 μg VLP-vaccinated pigs showed statistically less liver tissue fibrosis (p < 0.05) than that of the positive control pigs. Thus, the novel baculovirus expression system-generated VLP vaccine dose-dependently protects against HEV-3 challenge and may be useful in other animal species, including humans.
ISSN:2076-393X
2076-393X
DOI:10.3390/vaccines9111265